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After fixation, they were stained overnight at 4 C with Alcian blue 8-GX (Sigma, 1.0 % in 3 % acetic acid, pH 1.0)
Based on research using OptiMSM, it is an ingredient commonly found in exercise recovery products
Glutathione helps neutralize free radicals that trigger inflammatory responses
doi: 10.1126/scitranslmed.aad3740

The unbound conjugate is removed in a second separation and washing phase
