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[PMID: 2029805 DOI: 10.2165/00003088-199120020-00004] [DOI] [PubMed] [Google Scholar] 68.He R, Zheng W, Ginman T, Ottosson H, Norgren S, Zhao Y, Hassan M
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Tandem affinity purification of protein complexes from mammalian cells by the Strep/FLAG (SF)-TAP Tag
Immunoblotting and immunoprecipitation assays The cultured cells were rinsed once with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 mM HEPES (pH 7.0), 1% Triton X-100, 150 mM NaCl, 10% glycerol, 1 mM EDTA, 2 mM EGTA, 1 mM DTT, 5 mM Na 3 VO 4 , 5 mM NaF, 1 mM AEBSF, aprotinin (5 g/ml), and leupeptin (5 g/ml)
aeruginosa , Enterococcus faecalis , and E
