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Regulatory disclaimer PRODUCT USAGE: This PRODUCT IS INTENDED AS A RESEARCH CHEMICAL ONLY

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The kidneys have a very high concentration of mitochondria to fuel their constant filtration work

For K48- or K63-TUBE pulldowns, cells were lysed in 150 L of lysis buffer [100 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM EDTA, 1% NP-40, 0.5% Triton-X100, 10% glycerol] supplemented with 20 M MG132, 10 M PR619, 10 mM iodoacetamide, and a protease inhibitor cocktail (Nacalai Tesque) and were then extensively sonicated (Handy Sonic, TOMY Seiko)

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