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Cambrex, Milan, Italy) were grown in endothelial basal medium (EBM-2) containing 5% FBS (fetal bovine serum) and endothelial growth factor supplements (EGM-2 bullet kit, Cambrex)

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Importantly, much of HDLs (and apoA-Is) inhibition of macrophage activation is mediated through altering cholesterol levels in plasma membrane lipid rafts through cholesterol efflux mediated by ABCG1/SR-BI and ABCA1