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solubility, pH, and ionic strength should be validated per assay

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2.5.1.1 Small molecules analysis Compounds in the samples analyzed between 2020 and 2023 were identified using a high-performance liquid chromatograph (Ultimate 3,000 system from Dionex, Thermo Fisher Scientific, Waltham, MA, United States) coupled with a high-resolution micrOTOF-QII hybrid mass spectrometer (Bruker Daltonik, Bremen, Germany)