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The PCR protocol began with an initial denaturation step at 94 C for 3 min, followed by 35 cycles including a 1-min denaturation at 94 C, 1-min annealing at 58 C, and 1-min extension at 72 C

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performed a systematic review to describe the type of markers of oxidative stress identified in different biological specimens of patients with IPF [142]

[DOI] [PubMed] [Google Scholar] 191.Long Y.C., Zierath J.R