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The microplate was then incubated at 30 C for 30 min

[DOI] [PubMed] [Google Scholar] Riley 2011 Riley RD, Higgins JP, Deeks JJ

As mentioned in the Introduction, both lowered SpO2 and increased body temperature are part of the immune-inflammatory response, predict critical illness and death, and are manifestations of the infectious-immune-inflammatory core of acute COVID-19 [94]
The downside is that it costs more, and there are potential side effects

The enzyme reaction was followed by measuring the absorption spectrum of AuNPs with the reaction mixture