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doi: 10.1002/1873-3468.14022

IgA-Neph, IgA nephropathy (Bergers disease)

Despite the fact that it seems impossible to have animal models of psychiatric illness that fully reproduce the complex neurologic symptoms and associated comorbidities, animal testing for new drugs before clinical trials is an obligatory step

Lentiviral vector production and cell transduction Transfection mixture was prepared in 800 L OptiMEM (Gibco) by adding following plasmids: 12 g of psPAX2 (AddGene #12260), 5 g of pVSV-G (AddGene #138479) and 12 g of expression vector carrying shRNA sequence, in the presence of 80 L of TransIT- LT1 Transfection Reagent (Mirus, Mir2360)

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