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( 1 ) If one primer is designed to span an exon-intron boundary, the possible contaminating genomic DNA is not amplified, because the primer cannot anneal to the template

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1 Introduction Gentiopicroside (GPS), a secoiridoid glycoside, is extensively found in numerous traditional medicinal plants, including Gentiana scabra Bunge, Swertia chirayita, and Picrorhiza kurroa (Patel et al., 2019

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