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Although ROS generated under physiological conditions are not harmful, and likely play a signaling role, toxic or pathological conditions that lead to an impairment of mitochondrial function can increase the release of ROS

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The NdeI and EcoRI digested GSH1 or GSH2 cDNA was ligated into a NdeI/EcoRI digested pF1 plasmid to make pF1-GSH1-nsc and pF1-GSH2-nsc plasmids respectively