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Brunet, FOXO transcription factors
To introduce mutations to c-MET, short gene fragments (100150 nt) containing mutations were synthesized (IDT Inc.), and the corresponding gene fragment in the wild-type c-MET was replaced by the synthesized gene fragment using HiFi DNA assembly enzyme (NEB)
Reconstitute in water or acid, then dilute into buffer just before use
Characterization of the phthalate acid catabolic gene cluster in phthalate acid esters transforming bacterium- Gordonia sp
