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Western blot analysis and immunostaining demonstrated that NAC restored GS protein levels, while biochemical assays confirmed the recovery of glutamine concentrations in the culture media of astrocytes

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Furin is ubiquitously expressed

The hydroxylation reactions catalyzed by the PHD enzymes require molecular oxygen (O 2 ) in addition to the Fe 2+ and 2-oxoglutarate, therefore, reductions in oxygen content will result in loss of their activity