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References: Lenzi A, Lombardo F, Sgro P, et al

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475,476 In a study that included eight participants, the researchers let the experimental group perform twice-daily exercise, which was a 2-h muscle glycogen-depleting exercise followed by a 2-h rest and then the high-intensity interval exercise, and these twice-daily exercisers had a significant increase in the expression of PGC-1, compared to the once-daily and control groups