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Even at the highest temperature before we take the coffee out of the roaster, it has spent significantly fewer than 15 minutes from 200C to 230C
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Finally, the fermentation was scaled up in a 5-L fermentor, and the highest titer and yield were improved to 5680 mg/L and 45.13 mg/g, respectively, by optimizing the addition conditions of amino acids (40 mM added after 40 h)
