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In Na + titration experiments, saturating concentrations of carnitine were applied to oocytes expressing hOCTN2 and mutants (1150 mM NaCl, 150 mM - [NaCl] CsCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM HEPES, buffered to pH 7.4 with Tris base)

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The application of phosphorus nuclear magnetic resonance ( 31 P-NMR) spectroscopy revealed changes in energy metabolism in the brain of migraineurs [9,10,11,12,13]