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The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio

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In the ferroptosis inhibition group BSO&OXA@MOF-LR + DFO, ferroptosis inhibitor DFO was administrated via intraperitoneal injection

They stated that vitamin D deficiency may be a risk factor for ASD [99]

Important Considerations: Vitamin B12 shots are generally safe, but there may be mild side effects at the injection site