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A limitation of this method is that it requires the treatment of protein under anaerobic conditions, as TNB 2 readily converts to DTNB (Ellmans reagent) under aerobic conditions, leading to false positive identifications from free thiol labeling

Protocols, doses, and reactions reported below come from published clinical trials and self-reported community sources

Chen L, Hong W, Ren W, et al

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Regulation of HER expression and transactivation in human prostate cancer cells by a targeted cytotoxic bombesin analog (AN-215) and a bombesin antagonist (RC-3095) International Journal of Cancer