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Due to the mitochondrial membrane depolarization and membrane canals formation, the mitochondrial membrane permeability changes

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In a subset of patients recovered from COVID-19, long-term dysbiosis correlated with PASC symptoms, especially fatigue, joint pain, diarrhea, headache, depression, and anxiety 246

LPS Analysis LPS was analyzed by Limulus Amebocyte Lysate chromogenic assay (Lonza, Walkersville, MD) according to the manufacturer's instructions, with the following modifications: In the 104 CVID patients and 30 controls, samples were diluted 10-fold to avoid interference with background color, and preheated to 68C for 10 min prior to analyses to dissolve immune complexes as previously described (8, 17)