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Step 1: Preparation and Sterilization First things first

This article evaluates what the research actually shows

For the PD model, rats with unilateral 6-hydroxydopamine (6-OHDA) lesions in the substantia nigra were used to replicate PD pathology

In particular, immobilization (Negro-Vilar and Saavedra, 1980

Tendons and ligaments have limited blood supply compared to muscle
