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A representative fill is about 125 mg/mL total MIC plus ~1000 mcg/mL B12 in a 10 mL vial
It should also be emphasized that these ingredients may have drug interactions, which reduces the safety of pharmacotherapy
If endogenous lipid was retained, 45 ml of G20 buffer (200 mM NaCl, 20 mM imidazole, 20 mM TrisHCl, pH 7.5) was passed through the column and 175 units of thrombin (Avantor) were added to 5 ml of G20 to circulate over the column at 4C, overnight
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