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Animals and tissues In total, 40 male SpragueDawley rats (6 weeks old) were randomly allocated into five groups (n = 8 per group): control group, corn oil (MedChemExpress, China) injection [subcutaneously (s.c.)] + dimethyl sulfoxide (DMSO) (MedChemExpress, China) [intraperitoneally (i.p.)] + DMSO [intragastrically (i.g.)]

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The latter forces a continuous anabolic signal with very real downstream consequences

26 and the references therein), they have limitations associated with well-known multiple adverse reactions, genotoxicity, and special requirements for storage, handling, and administration, posing challenges with respect to the standard operating procedure (SOP) for MDA campaigns requiring single or double dose activity, oral administration, minimal toxicity, and stability in tropical settings 27

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