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Longevity pathways converge on autophagy genes to regulate life span in Caenorhabditis elegans

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From the mixture, 10 l were aliquoted, loaded on each side of an improved Neubauer hemocytometer, and the hemocytes were counted under a light microscope (Leica DM 2500 LED, Leica microsystems, Wetzlar, Germany)

Future prospective studies should use more extensive fatigue questionnaires, such as the Fatigue Assessment Scale (FAS) (64) or the 11-point fatigue numeric rating scale (NRS) (65, 66)