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Curcumin inhibits the phosphorylation of Raf kinase, thereby blocking MEK1/2 from phosphorylating ERK1/2, which in turn inhibits the activation of ERK1/2 (Sun et al., 2017
ab178860, Abcam) overnight at 4 C followed by incubation with a horseradish peroxidase-linked secondary antibody
Visser C, Biedermann JS, Nierman MC, van der Meer FJM, Gulpen AJW, Moors YCF, et al
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