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Step 4: Let the water trickle slowly down the vial wall

Tumour tissues (100500 mg) were dissected into smaller fragments to cover the filter surface of 0.22 m nylon-filtered microcentrifuge tubes (Corning, 8160), taking care to minimize mechanical cell lysis

Avoid injecting into the same spot repeatedly

[DOI] [PMC free article] [PubMed] [Google Scholar] 66.Armeni T., Cianfruglia L., Piva F., Urbanelli L., Luisa Caniglia M., Pugnaloni A., Principato G

The catch is the complete absence of human trials and long-term safety data
