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The gradient was as follows: starting at 10% B and increasing to 45% B over 2.7 min, then to 53% over 0.1 min, to 65% over 6.2 min, to 89% over 0.1 min, to 92% over 1.9 min and finally to 100% over 0.1 min

(2015) used acrolein as a selective agent to isolate glutathione-overproducing strains through adaptive laboratory evolution (ALE) under gradually increasing concentrations