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Quantitative RT-PCR was performed on LightCycler 480 (Roche) using the LightCycler 480 High Resolution Melting Master Mix (Roche) and the following primers: cbiL-forward: 5-GCGCGAGGCAGACGTGATCC-3, cbiL -reverse: 5-GACACCGGACCTCTCCCGCA-3, cysG+cbiX-forward: 5-TGTATTCCGCCCCGCTGTTGC-3, cysG+cbiX-reverse: 5-GAGCACTGCCGACGTGTCCC-3, btuR-forward: 5-GGAAGATGCTCTTCGGGCGCT-3, btuR -reverse: 5 -GCCTCAGGGTTCTCCGCAGC-3, 16S-forward: 5-GGGGCTTAACCCTGAGCGTGC-3, 16S-reverse: 5-TTCGCTCCCCACGCTTTCGC-3

This makes sense physiologically

[5] [10] In 2023, it was the 104th most commonly prescribed medication in the United States, with more than 6 million prescriptions

Alcohol is metabolized by the liver and messes with the filtration as well

Cyanocobalamin : This is the of vitamin B synthetic produced form 12 commonly found in supplements and fortified foods