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Oncogenic Raf-1 disrupts epithelial tight junctions via downregulation of occludin

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For cellular fractionation, the tissues were homogenized in H-medium (70 mM sucrose, 220 mM mannitol, 2.5 mM HEPES, 2 mM EDTA, 0.1 mM phenylmethylsulfonylfluoride, pH 7.4) at 4C and the fractions separated by differential centrifugation and the purity of the fractions were checked as described before (Raza et al., 2004

Different redox sensitivity of endoplasmic reticulum associated degradation clients suggests a novel role for disulphide bonds in secretory proteins