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To obtain a ctl-2 mutant with GFP reporters, we crossed GFP-carrying strains (OG497 and VS15) as follows: unsynchronized populations abundant in L4 stage worms of GFP-carrying strains were repeatedly heat shocked at 30C or 37C for multiple generations until male worms were observed on the plate

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Le tatouage des lvres ou maquillage permanent des lvres seffectue sur le contour des lvres ou sur les lvres, en insrant le pigment de couleur afin de marquer sa couleur et son contour