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VC-S and VDA performed the HPLC assays, analyzed data and reviewed the manuscript

DNA protection (water-soluble antioxidants like vitamin C scavenge ROS in the seminal plasma, reducing oxidative DNA damage)

Technical Characteristics Compound name: 5-Amino-1MQ Type: Synthetic compound (non-peptidic) Form: Powder Purity: 98% Regulatory status: Research Use Only (RUO) Classification: Not intended for clinical, therapeutic, or diagnostic use Each batch is tested to ensure identity, purity, and consistency, ensuring reliability for scientific research applications

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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C