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Illumina sequencing purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina Nextseq2000 platform (San Diego, USA) according to the standard protocols Technology Co

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For example, you could increase a clips speed by 200 percent, then reverse it and then play forward at 50 percent all within the same clip, with only one effect applied

Full MS spectra were acquired at the resolution of 140,000 at m/z 200, scan range 3801200 m/z (negative ion mode, 017 min) and 3801200 m/z (positive ion mode, 1734 min), AGC target 1e6 counts, Maximum IT 100 ms

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