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Slides were cut for each biopsy in 5 m sections, and stained with hematoxylin and eosin to confirm menstrual cycle phase using the Noyes criteria 43

In the pivotal 2018 animal study by Neelakantan et al., systemic treat Mechanism of Action 5-Amino-1MQ inhibits NNMT (nicotinamide N-methyltransferase), an enzyme that catalyzes the N-methylation of nicotinamide using S-adenosylmethionine (SAM) as the methyl donor, producing 1-methylnicotinamide (MNA) and S-adenosylhomocysteine (SAH) as products

This causes depressurization of the NP and inward collapse of AF fibers, which causes degeneration similar to annular disc herniation

For immunohistochemical staining, the tissue sections were inactivated by endogenous peroxidase with 3% hydrogen peroxide for 10 min and blocked with goat serum for 30 min

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