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Free radicals such as ROS and NO, as well as cytokines in the inflammatory microenvironment, can interfere with the normal function of the ER, promoting abnormal protein folding within the ER and exacerbating ERS (Hasnain et al., 2012)

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The primary hypothalamic cells expressing glucokinase are within the arcuate nucleus, ARC

Immunofluorescence Fibroblast cultures at passage 3 were cultured in eight-well chamber slides (Gibco, Burlington, ON, Canada) in growth medium (DMEM, 10% fetal bovine serum (FBS), penicillin, and streptavidin from Invitrogen, Burlington, ON, Canada)

After 1821 days of occlusion, the concentration of HSP70 was lower than in the control