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These data indicate that we developed a NE primary cell culture in which we could observe the activity of NET in endogenous cells

Additionally, the potential role of PPAR in trophoblast differentiation and placental development is also highlighted [24, 30], and the activity of PPAR can be deacetylated and regulated by SIRT1 through recruiting cofactors, such as NCoR1 (nuclear receptor corepressor 1), SMRT (silencing mediator of retinoic acid and thyroid hormone) and Prdm16 (PR domain-containing protein 16) [31, 32]

& Bhattarai, S

lncRNA MIR100HG-derived miR-100 and miR-125b mediate cetuximab resistance via Wnt/-catenin signaling

Finally, the cover slips were washed four times with PBS containing 0.05% Tween 20 (PBST), incubated with either goat anti-rat or goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor Plus 488 (Invitrogen) for 1 h at room temperature, washed with PBST, and mounted on ProLong Gold Antifade Mountant with DNA Stain DAPI (Invitrogen)