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Mechanisms underlying drug-induced ototoxicity The possible mechanisms by which ototoxic agents can influence auditory function are nerve demyelination, apoptosis via caspase cascade, glutamate toxicity, ROS overload, DNA/RNA damage, inhibition of mitochondrial protein synthesis, inner ear hypo-perfusion and ion disturbance on the background of gene mutations, and increased permeability of triggered drugs (Fig

In contrast, targeting the adeno-associated virus integration site 1 locus ( AAVS1 ) did not affect the levels of IRFP670-positive cells and hence served as negative control

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