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Harvested cells were dissolved in sterile water and lysed by bead beating using 0.1 mm Zirconium beads
This creates a catastrophic vulnerability within the cells
Our group has recently invented a molecular combination (cARLA) synergistically targeting signaling pathways related to BBB maturation that elevated the barrier tightness and other BBB-specific properties in several BBB culture models including human stem cell-based ones [40]
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Clin Biochem 2000
