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Nimmo and Philip Cohen reported purification of protein phosphatase inhibitor-1 (PPI-1) from rabbit skeletal muscle utilizing an approach that includes heat treatment at 90 C as one of the purification steps (Nimmo and Cohen, 1978)

10.2174/1381612826666200424180139 68 SikiriP.PetekM.RucmanR.SeiwerthS.GrabareviZ.RotkviI.et al (1993a)

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Bone marrow cells were processed for CD34 + cell selection, transduced with the HPV569 lentiviral vector and thawed for release testing