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The unreacted substrates acted to reduce the Cu(II)neocuproine complex into the strongly coloured Cu(I)neocuproine complex that could be measured spectrophotometrically at 450 nm

Assay Reaction: Prepare the reaction mixture by combining the GPx Assay Buffer, GPx Substrate, GPx Enzyme, Glutathione Reductase (GR), and NADPH

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Administer as Directed:Only inject or infuse the resultant solution per your providers or medications guidelines
