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The membrane was then incubated with Notch1(Santa Cruz Biotechnology, CA, USA, sc-376,403), Notch2(Cell Signaling Technology, Danvers, MA, USA, 4530), Notch3(Santa, sc-5593), Notch4(R&D, MAB3847), Hes1(Millipore, NG1839542), Hey1(Millipore, NG1829781), Itch(Santa, sc-28,367), CRMP5(Santa, sc-58,515), P21(Abcam, Cambridge, UK, England, ab109520), CDK2(Abcam, ab32147), Cyclin E (Abcam, ab33911), Bax(Cell Signaling Technology, 5023), Bcl-2(Cell Signaling Technology, 15,071) or -actin(Proteintech, Wuhan, China, 60,0081-lg) respectively at 4 C overnight and followed by a secondary anti-rabbit or anti-mouse antibody (Cell Signaling Technology) at room temperature for 1 h

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With cross-sectional studies correlating mushroom consumption with better brain function, researchers decided to put it to the test using lions mane mushroom, which is especially popular in traditional Chinese medicine