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Its mechanisms primarily involve glutathione (GSH) depletion, inactivation of glutathione peroxidase 4 (GPX4), accumulation of lipid peroxides, and high levels of free iron that bind to polyunsaturated fatty acids, which trigger the Fenton reaction to release excessive ROS [119]

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In the early stages (definitive endoderm and foregut stages) of differentiation, cells rely more on glycolysis for energy production and gradually start transitioning into OXPHOs state as the mitochondrial machinery remodel