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However, this review also noted that the quality of the studies was not very high, so more studies are needed before its possible to make any firm recommendations

Conditions where the bone marrow overproduces blood cells, including polycythemia vera, chronic myeloid leukemia (CML), and hypereosinophilic syndrome , can cause elevated B12 levels

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20 L of this dilution were mixed with 10 L of 4X Laemmli SDS buffer (400 mM DTT, 8% SDS, 200 mM Tris-HCl, pH 6.8, 40% Glycerol, 0.02% bromophenol blue) and denatured at 95 C for 5 min