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After DNase I treatment, the soluble fraction was mixed with Dynabeads Protein G (Life Technologies) coupled with mouse monoclonal anti-Pk antibody (BioRad) and incubated at 4 C for 2 h

For the construction of calibration curves, a series of dilutions of Hcy, cystathionine, cysteine, GSH, and GSSG mixtures were prepared just before use at the concentrations of 0.01, 0.1, 1, 10, 50, and 100 M

doi: 10.1186/s13104-021-05700-x 418 PrietoKCaoYMohamedETrillo-TinocoJSierraRAUrueaCet al

Evidenced through scanning electron microscopy, the cell structures of Caco-2 cells (cultured enterocytes) were well protected from the attack of the B

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