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Destructive role of TMAO in T-tubule and excitation-contraction coupling in the adult cardiomyocytes

After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Fisher Scientific, 1:5000), the proteins were visualized using chemiluminescence reagents (29050, Engreen, San Jose, CA, USA) with a Gel Doc EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed using the Image Lab software (Bio-Rad Laboratories)

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the respiratory chain in the mitochondria therefore carries out a kind of biochemical detonating gas reaction, whereby the respiratory substrates are dehydrogenated

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