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For preparations made at pH 7.5 which had predominantly diglutathionylated M pro the preparation was incubated at 37C for 30 minutes in the presence of buffer control (50 mM Tris, pH 7.5, 2 mM EDTA, and 100 mM NaCl containing 100 ug/ml of cm-BSA), Grx (350 nM) alone, GSH alone (0.5 mM) and Grx and GSH together

Since an adverse reaction can arise up to several weeks following disulfirams discontinuation, ongoing caution (and abstinence) is warranted

doi: 10.1093/gigascience/giy111 58 ZhaoX.DrlicaK

Lorenzo Patricia February 18, 2022 at 11:08 am - Reply Hi Lorenzo