glutathione cycle in mitochondria Glutathione-depleting mitochondria-targeting nanodrugs for stress amplification and immune activation via synergistic photodynamic therapy and cuproptosis Glutathione (GSH) and antioxidant enzymes,

study used intra-articular injection under clinical supervision

knocking down ELAVL1 alone noticeably decreased proliferation, migration, and invasion, but combining ELAVL1 and GAC knockdown (which depleted both glutaminase isoforms) did provide a small further decrease in cell growth, migration and invasion compared to that with ELAVL1 knockdown alone

Kim SY, et al

At the end of the experiments, the mice were sacrificed (cervical dislocation for short distribution time points and carbon dioxide asphyxia for longer ones), and the draining LNs (ipsilateral popliteal), the second LN in the chain (lumbar), and nondraining LNs (axillary and contralateral popliteal LNs) were rapidly dissected free (Figure 1A)

Even lyophilized powder shouldn't be left out for extended periods