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Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set the counter to zero with distilled Preheat Reagent II in water bath for 30 minutes: 37 (mammal cell) or 25 (other species)
Notably, around 60% of proteins in the secretomes of untreated fibroblasts overlapped with those of untreated SKOV3 cells
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doi: 10.1155/2013/825928 87 MostJGoossensGHJockenJWBlaakEE
