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Highlights: RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage Easily quantitate GSH levels in live cells using confocal microscopy/FACS Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification

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This metabolic division allows pathogens to balance the need for energy production while meeting the biosynthetic demands of replication within the host cell

Liver homogenates (10 L) were protein-precipitated with nine volumes of acetonitrile/methanol/formic acid (74.9:24.9:0.2, v/v/v) containing stable isotope-labeled internal standards (valine-d8 and phenylalanine-d8)