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The vector was linearized with PacI and was cotransfected with pACAd-9.2-100 into 293AD cells (Cell Biolabs, AD-100) according to the manufacturers protocol (Cell Biolabs, VPK-254)

As for women, a randomized, doubleblind, placebocontrolled clinical trial involving 150 women (aged 18-39) with clomiphene-resistant PCOS found that adding NAC (1.2 grams/day for five days starting on day three of the cycle) to clomiphene citrate significantly increased both ovulation and pregnancy rates

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Plant Genome (2017)

Sharpe et al