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[DOI] [PMC free article] [PubMed] [Google Scholar] 300.Zhang Y, Chen M, Dai Z, Cao H, Li J, Zhang W

C., Padhi, M

Lentiviral production and silencing in ECs Lentiviral vectors containing a KLF2-expressing construct, in which the human KLF2 gene was inserted downstream of the PGK promoter in the pRRL-cPPT-PGK-MCS-PRE-SIN backbone, a short hairpin against CBS (shCBS, 5-CCGTCAGACCAA GTGGCAAA-3), CTH (shCTH, sigma, TRCN0000078263) or a scramble as a control (pLKO, Addgene #17920), were produced in HEK293T (ATCC, CRL-11268) cells with packaging vectors pMD2.G (VSV-G envelope, Addgene #12259), pMDLg/pRRE (Gag/Pol, Addgene #12251), and pRSV-Rev (Rev, Addgene #12253) by polyethylenimine (PEI)-mediated transfection

B12 is a nutrient your body uses for normal energy metabolism, red blood cell formation, and nervous-system function

doi: 10.1038/s41584-020-0419-z 62 LiuBLiJChenBShuaiYHeXLiuKet al
