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Cells were cultured in Leibovitzs L-15 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) containing 15% fetal bovine serum (Biochrom AG, Berlin, Germany), 50 U/mL penicillin/streptomycin (Gibco), and 50 g/mL gentamicin (Gibco)

Studies have found that the GSH content of hippocampal neurons is reduced, the oxidation level is increased, and the susceptibility to oxidative damage is increased in EAAT3 knockout mice

Moreover, further studies are needed to define the impact of vitamin D on MS-related fatigue and to optimize suitable doses and treatment times

Your cells can regulate glutathione production based on their specific needs, creating glutathione precisely where it's needed most

Nutrients 14 (12), 2421 (2022)