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Sperm DNA fragmentation measured by sperm chromatin dispersion impacts morphokinetic parameters, fertilization rate and blastocyst quality in ICSI treatments
For cells treatments, AOAA (Selleck Chemicals LLC, Houston TX 77230 USA), I3MT-3 (MedChemExpress, Sollentuna, Sweden ) , and Erastin ( Tocris-Bio-Techne SRL, Milano, Italy) were solubilized in DMSO
Tissue sections were incubated with primary antibodies (Supplementary Table 2) overnight at 4 C
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After washing with PBS, the proportion of BMDCs that phagocytosed FITC-labeled 4T1 cells (CD11cFITC cells) was analyzed using flow cytometry
